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BioCurious Library

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Saved by anithabodepudi
on May 11, 2012 at 10:22:02 pm
 

Modern cloning methods which do not use restriction enzymes


LIC cloning 

 

Allows the creation of a plasmid from a single T4 DNA polymerase reaction from a plasmid and PCR reaction product. 

http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/LIC_cloning

 

SLIC, Gibson and CPEC cloning methods 

http://j5.jbei.org/j5manual/pages/22.html

 

Gateway Cloning

creates multiple plasmids from a single template plasmid. 

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