Modern cloning methods which do not use restriction enzymes
LIC cloning
Allows the creation of a plasmid from a single T4 DNA polymerase reaction from a plasmid and PCR reaction product.
http://strucbio.biologie.uni-konstanz.de/ccp4wiki/index.php/LIC_cloning
SLIC, Gibson and CPEC cloning methods
http://j5.jbei.org/j5manual/pages/22.html
Gateway Cloning
creates multiple plasmids from a single template plasmid.
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